Burkhard Rost is a scientist leading the Department for Computational Biology & Bioinformatics at the Faculty of Informatics of the Technical University of Munich (TUM).

[7] Following research internships at EMBL and the European Bioinformatics Institute in Cambridge (UK), in 1998, he became assistant professor at the Department of Biochemistry and Molecular Biophysics in the College of Surgeons and Physicians of the CU Medical Center of Columbia University in the City of New York.

Examples of research carried out in his lab includes the prediction of enzymatic activity (ECGO), interaction partners (ISIS, DISIS, PiNAT), subcellular localization (LOCtree, LOCnet, PredictNLS), functional effects of point mutations/SNPs (SNAP), disordered regions (MD, NORSnet, Ucon), membrane spanning segments (PROF/PHDhtm), secondary structure (PROF/PHD, RePROF, DSSPcont),[17] solvent accessibility (PROF/PHD, RePROF), internal residue-residue contacts (PROFcon) and the clustering of proteins into families (CHOP).

His group has been dedicated to making their tools available online as demonstrated through the first internet server for protein structure prediction and sequence analysis, Predictprotein,[6] that was launched in 1992, and has been continuously in service ever since.

Rost's work has been published in leading peer reviewed scientific journals including Nature,[8][19][20][21] Science,[22] PLOS Genetics.