[3] Other distinguishing features include the slightly lighter face mask and shorter appearance of the California myotis' tail.

[2] The California myotis is largely free of ectoparasites commonly found on other bat species, such as fleas, ticks, flies, lice, and bed bugs.

In coastal regions, the California myotis favors riparian groves with sycamore, willow, and cottonwood trees.

[4] During the day, the California myotis will roost in the bark of dead trees,[6] particularly ponderosa pines,[6][7] rock crevices, or buildings.

The California myotis prefers forested areas and is seldom found in urban environments or open rural spaces.

For the remainder of the year, the adults of the two sexes prefer to remain separate, occasionally roosting together during the months of September, October, February, and March.

During the birthing season they form small maternity colonies of about 20 individuals, usually in the loose bark of trees or rock crevices.