It ran 3.86 miles (6.21 km), from the ferry at Kaighn's Point in Camden on the Delaware River across Newton Creek to Gloucester City.

A 1.2-mile (1.9 km) extension from Gloucester City to Mount Ephraim, New Jersey, opened in May 1876.

[3][4][a] The Philadelphia and Reading Railway acquired stock control of the Camden, Gloucester and Mount Ephraim Railway in 1884 and took steps to coordinate its operations with that of the Philadelphia and Atlantic City Railroad, a 3 ft 6 in (1,067 mm) railroad running between Camden and Atlantic City, New Jersey.

[3] The Reading converted the Philadelphia and Atlantic City Railroad to standard gauge on October 5–6, 1884.

A connection was built to allow Philadelphia and Atlantic City Railway trains to reach the ferry at Kaighn's Point.