Occupying several buildings within the Central Experimental Farm, the museum operates as a "working farm", and provides public programs and exhibitions on agriculture sciences, and on the history of agriculture in Canada.

Initially occupying only a dairy barn, the museum later expanded to include several other buildings on the Central Experimental Farm.

[4] In 1978, exploratory discussions took place regarding the feasibility of creating an agricultural museum within one of the heritage buildings of the Central Experimental Farm.

[4] In 1980, the upper floor of the dairy barn was selected to host an exhibition gallery for the collection.

[9] Several other buildings on the property are also used by the museum as event spaces, including the "Meadowview Barn/Building 95" and the "Cereal Barn/Building 76".

The Meadowview Barn was erected in 1912 to support dairy research, and is used by the museum to host special events and summer camps.

[10] The museum is operated by Ingenium, a crown corporation responsible for preserving and protecting Canada's scientific and technical heritage.

[4][14] In addition to exhibitions on the history of agriculture, the museum also holds a historical collection of tractors.