However, in 1624, after controversies over her right to possession of the territory raised by another heir, Álvaro Pires de Castro e Sousa, Count of Monsanto, Mariana was removed from her position.

These expeditions were responsible for creating settlements in territories that are today located in the interior of São Paulo, such as Taubaté and Sorocaba, and for exploring regions that later became the states of Paraná, Minas Gerais, Goiás and Mato Grosso.

[5][4] Over the next few decades, groups of explorers searched for treasures in the backlands of today's Minas Gerais, in the areas of the captaincies of Itanháem and São Vicente.

When gold was found, prospectors from other places in the colony and even abroad disputed the ownership and exploitation of the mining region, which led many explorers from Itanhaém to ally with those from São Vicente in a battle against the outsiders, resulting in the War of the Emboabas.

They fought in court with the holders of the family of the Count of Monsanto, trying to regain possession of the Captaincy of São Vicente, in a process that lasted more than a century.