[4] Carlie Cooper first appeared in The Amazing Spider-Man #545 (December 2007) and was created by Dan Slott and Joe Quesada.

[8] She later discovers that the "Spider-Tracer Killer" is a conspiracy by a group of New York cops who have planted the tracers on dead bodies to frame Spider-Man in an attempt to turn the public against.

Carlie informs the precinct's sergeant, who is secretly a part of the conspiracy and issues a warrant for her arrest, claiming that she is aiding and abetting the killer.

After several near misses and almost-dates, Carlie and Peter Parker finally set up a real date at the Coffee Bean.

Harry and Mary Jane Watson happen to be there and the two girls get talking, when all of a sudden Lily bursts through the wall, followed by an attacking team of supervillains.

[17] The police arrive and Tombstone escapes but later doubles back to follow her after discovering that Carlie knows Menace's secret identity.

It is at this time that Peter discovers Carlie is a roller derby participant, using the sport to help her unleash any pent up anger.

[20] When Peter is called out on a mission with the Future Foundation, Carlie is told it's a business trip for Horizon Labs.

After getting drunk, Carlie decides to get a tattoo of the Green Goblin's insignia out of spite, knowing Peter's opinion of Norman Osborn.

[1] During the "Return of Anti-Venom" storyline, Carlie correctly deduces that the new Wraith is her police captain Yuri Watanabe using a mask Mysterio created that could impersonate the face of the late Jean DeWolff since only they and Spider-Man knew about it.

This becomes more obvious when she visits evidence storage and learns of a mysterious incident that supposedly destroyed the mask with Watanabe being the last visitor beforehand.

Having secretly placed her cell phone into Watanabe's pocket earlier and following her target, Carlie's suspicions are confirmed.

[25] She and the rest of the civilian population were eventually cured when Spider-Man uses Doctor Octopus's octo-bots to disperse the antidote.

[26] Carlie is then seen hiding behind a tree in Central Park, cured but naked due to her cloths having been ripped off.

[4] However, she and Peter are on a sort of truce as she requires Spider-Man's help in order to investigate the death of a gothic teen who died from falling from a great height and was ruled as a suicide.

[38] At the time when the Goblin Nation was causing havoc in Manhattan, Carlie attacks Parker Industries where Octavius gets her away from the other people inside.

The Superior Spider-Man deploys Living Brain to fight Monster while Octavius and Sajani Jaffrey flee inside.

After tells Sajani to split up wanting to direct Monster's attention, Octavius fights Carlie in a closed room.

[40] In the aftermath, as she was still recovering from the effects of the Goblin serum, Carlie has one last talk with Mary Jane where she has decided to leave New York since she realized that she will always remain a target as long as she remains close to Peter or Spider-Man and wants to move to a less dangerous place where she could work as a police forensic officer without having to deal with any supervillains.

She catches up with Mary Jane in a coffee shop and reveals she did lab work down in New Orleans before deciding to returning to New York.

Noticing that Mary Jane is dating Peter once more, she decides to introduce a support group featuring friends and loved ones of superheroes called "The Lookups".

Carlie was mutated into a spider monster alongside Mary Jane Watson, Betty Brant, and Sharon Carter by the Queen and used as bait by the heroes.