Caroline Le Souef (15 July 1834 – 6 March 1915) was an Australian artist who was born in England in 1834 to John Cotton.

[1] As a child, she migrated to Australia with her family in 1843,[2] living in Goulburn River Valley, and was educated in Melbourne, Victoria.

Caroline would provide sketches and artworks to complement the work of Albert Le Souef during their time in the Goulburn River Valley when the treatment of Aboriginal people was conducted under a Protectorat, with Albert taking an active role.

Albert and Caroline would document the customs of the local Aboriginal people during this time.

After Albert was appointed foundation director of the Melbourne Zoological Gardens in 1880, the pair toured Europe, and she has a number of sketches attributed to the collection of specimens around this time.