First unveiled in early 1999, it serves as a collection of songs from the channel's programs and anthological series, including those from Hanna-Barbera and others like Cow and Chicken and The Powerpuff Girls.

Critically, Cartoon Medley was awarded three out of five stars by AllMusic while "The Powerpuff Girls (End Theme)" was singled out by Billboard's Moira McCormick for being a "breakout" track.

The CD's cover advertises containing "Flaky fried Cow and Chicken, Space Ghost-Zorak-Brak veggies, and [a] Yogi gelatin treat".

[2] While creating the compilation album, Cartoon Network's Vice President of Off-Channel Commerce, Jamie Porges, imagined having more material than just the title songs from television series.

[9] The themes from Hanna-Barbera's series on Cartoon Medley were selected from Josie & the Pussycats, The Flintstones, The Jetsons, Dastardly and Muttley in Their Flying Machines.