She attended Brown University and received a dual degree in Art/Semiotics and Women's Studies,[5] and was a member of Phi Beta Kappa.

[7][8] Much of her early video work from this time is held at the New York Public Library as a part of their AIDS Activist Videotape Collection.

Her work in the early 1990s included Bleach, Teach, and Outreach (1989, co-produced with Ray Navarro) to document the emergence of a city-sponsored needle exchange program to combat the spread of HIV; Keep Your Laws Off My Body (1990, co-produced with Zoe Leonard) about censorship and legislation against privacy and lesbian bodies; Among Good Christian Peoples (1991, co-produced with Jacqueline Woodson) based on Woodson’s humorous essay about growing up as a Black lesbian Jehovah’s Witness; I’m You, You’re Me: Women Surviving Prison Living with AIDS (1992, co-produced with Debbie Levine); Sacred Lies, Civil Truths (1993, co-produced with Cyrille Phipps) documented the Religious Right and its broad-based agenda, analyzes their campaigns for anti-gay initiatives in Oregon and Colorado in 1992, also examining issues of family and religion in lesbian and gay communities; Not Just Passing Through (1994, co-produced with Polly Thistlethwaite, Dolores Perez, and Jean Carlomusto) a four-part documentary about constructions of lesbian history, community and culture; Cuz It's A Boy (1994, about the murder of Brandon Teena); Positive: Life with HIV (1993-95, senior associate producer & segment producer) AIDSFILMS’ four hour series about HIV/AIDS targeted at the HIV community covering political, psycho-social, cultural, medical and legal issues of living with HIV/AIDS.

[9] In 1996, Gund founded Aubin Pictures, a nonprofit documentary film company with scholar and activist Scot Nakagawa.

In 2009, Gund produced and directed a segment for Sesame Street called Rhyme Time (2009) with poet Idris Goodwin about kids and healthy eating.

(2009), a documentary directed by Gund and two eleven-year-old girls about healthy, sustainable eating from a kid's perspective, premiered at the Berlin International Film Festival and was featured in the Discovery Channel's Planet Green.

The film chronicles Agnes Gund's stunning journey to sell a Roy Lichtenstein painting to invest in the Art for Justice Fund.

Gund produced Primera (2021) for HBO that told the story of four parents-turned activists leading Chile's revolutionary path to a new constitution.