Chamberlayne is a census-designated place (CDP) in Henrico County, Virginia, United States.

[5] As of the census[2] of 2000, there were 4,380 people, 1,884 households, and 1,352 families residing in the CDP.

The racial makeup of the CDP was 47.83% White, 49.20% African American, 0.16% Native American, 1.12% Asian, 0.09% Pacific Islander, 0.39% from other races, and 1.21% from two or more races.

There were 1,884 households, out of which 19.5% had children under the age of 18 living with them, 58.4% were married couples living together, 10.0% had a female householder with no husband present, and 28.2% were non-families.

24.2% of all households were made up of individuals, and 12.1% had someone living alone who was 65 years of age or older.