His duchy was therefore inherited by his granduncle Philip II (reigned 1496–1497), the male heir of the Savoy line.

Charles's heir-general was his underage sister Violante Ludovica, who was married to Philip's eldest son Philibert the Handsome.

Violante however died in 1499, 12 years old and childless, leaving the 18-year-old Philibert (who had succeeded his elderly father as Duke in 1497) a widower.

Charles's and Violanta's next heir general was their first cousin Princess Charlotte of Naples (1479–1506), daughter of their aunt Anne of Savoy.

Their female descendants claimed the Kingdom of Jerusalem, although de jure their claims to the Kingdom of Cyprus, Jerusalem and Armenia (as well as the rights of René of Valois-Anjou) belong to the line of the heir general, not to the line of Philip II, Duke of Savoy.