The Charleston Southern Buccaneers are the athletic teams that represent Charleston Southern University, located in North Charleston, South Carolina, in intercollegiate sports at the Division I level of the National Collegiate Athletic Association (NCAA), primarily competing in the Big South Conference since the 1983–84 academic year.

Charleston Southern competes in sixteen intercollegiate varsity sports.

Men's sports include baseball, basketball, cross country, football, golf, and track and field (indoor and outdoor); while women's sports include basketball, cross country, golf, soccer, softball, tennis, track and field (indoor and outdoor), and volleyball.

These two schools first met on the football field in 2003 and it has been a rivalry since Charleston Southern defeated Coastal Carolina 34–27 in 2005 to win a share of the Big South Championship that Coastal had already clinched.

Charleston Southern won a share of the 2016 Big South Championship and got the automatic berth into the NCAA Playoffs.