Her maternal grandparents were John IV de Longwy, Baron of Pagny, and Jeanne of Angoulême, a natural (but legitimated by Royal decree in 1501) half-sister of King Francis I of France.

By some accounts, Charlotte's father determined to thwart his wife's influence by sending three of his daughters to convents.

[1] Her father, influential in the court of Catherine de' Medici, placed her in the royal convent of Jouarre, near Meaux, to be raised as a nun.

[2] Other sources claim that Louis simply wanted to avoid paying dowries in order to conserve his only son's patrimony.

[7] Following her death, William married on 24 April 1583, his fourth and last wife, Louise de Coligny, by whom he had a son Frederick Henry, Prince of Orange.