ChiRP-Seq

Over the last few years lncRNAs have been the least explored and functionally characterized emerging regulatory molecules, especially in comparison to their short counterparts, small ncRNAs.

[1] ChiRP-Seq is a new technique that has allowed researchers to map long RNA occupancy across the genome at a higher resolution than ever before.

[2] This technique will allow scientists to generate a map of genomic binding sites of several hundred bases very accurately due to high sensitivity and low background.

Complexes containing biotin-probe + RNA of interest + DNA fragment are captured by magnetic beads labeled with streptavidin.

For example, genomic targets of enhancer RNA which act at a distance from their site of synthesis can be easily evaluated by ChiRP-Seq.

Overview of a next generation sequencing method to characterize RNA binding sites to chromatin. Chromatin isolation by RNA purification.