The Chicago, St. Louis and New Orleans Railroad was a 19th- and early-20th-century railway company in Kentucky in the United States.
In 1896, it purchased the Chesapeake, Ohio and Southwestern Railroad and those former rights-of-way currently form parts of the class-II Paducah and Louisville.
In 1913, it purchased the Paducah Union Depot Company and the Kentucky Valley Railroad.
The Chicago, St. Louis & New Orleans connected with the Owensboro and Nashville Railway (and later the L&N) at Central City in Muhlenberg County.
In 1877, Stuyvesant Fish was elected secretary of the Chicago, St. Louis and New Orleans Railroad company.