Not long after she made plans to move to Los Angeles, Pengilly was invited for a screen test and she was offered the part of Chloe a week later.

Chloe comes into conflict with her family, neighbours and boss when she launches an escort service, which sees her charging men for platonic dates.

For her portrayal of Chloe, Pengilly was included on the long list for the Logie Award for Most Popular New Talent, despite not having appeared on-screen when voting opened.

[2] The character is the sister of the established Brennan brothers; Mark (Scott McGregor), Aaron (Matt Wilson), and Tyler (Travis Burns) who were introduced in 2010 and 2015 respectively.

"[4] On 1 March 2018, Alison Stephenson for The Daily Telegraph confirmed that actress and model April Rose Pengilly had been cast in the role of Chloe.

[9] After dying her hair blonder for the role, Pengilly spent six months posting photos of herself as a brunette on social media and setting Sydney as her location.

"[3] The show's executive producer Jason Herbison said the crew were "delighted" to introduce Chloe and praised Pengilly's casting, saying she "brought a fantastic energy to the character".

[10] The character's look is "casual feminine boho chic", a style worn by actresses Teresa Palmer and Sienna Miller.

"[6] In her fictional backstory, Chloe was in her early teens when her parents' separated, and she was raised away from her father and brothers by her mother Fay Brennan (Zoe Bertram) in Adelaide.

[6][9] Pengilly felt like she had real brothers in McGregor and Wilson, as they were very welcoming towards her when she started, and she said her favourite scenes to film were set in the Brennan house.

[14] The storyline begins when Leo is ordered by Terese Willis (Rebekah Elmaloglou) to fire Chloe for incompetency, after she evicts lawyer Toadfish Rebecchi (Ryan Moloney) from his office within the Lassiters complex.

[14] A Neighbours publicist told Claire Crick of Soaplife that "Leo realises that maybe there's more to bolshy Chloe than meets the eye and he tries to seduce her.

[16] He also admitted that some of the scenes between the characters may not have made it past the censor, and he was interested to see how much would have to be edited for the show's early evening time slot.

[16] In a later interview, Kano said Leo was capable of developing genuine feelings for Chloe despite the initial fun and casual nature of their relationship.

[20] Prior to the diagnosis, Chloe receives several letters and emails from a clinic in the United States, where she underwent some genetic medical trials in return for money.

[22] Pengilly also enjoyed exploring another side to her character, but she preferred the lighter scenes where Chloe is the comic relief, saying "comedy is my jam".

[20] Chloe struggles in the wake of her diagnosis and takes a sick day from work, before inviting Amy Williams (Zoe Cramond) on a girls night out.

[25] Pengilly told Sarah Ellis of Inside Soap that she did not see the storyline coming, but she understood that her character was desperate to pay back her mother.

[27] Orion's friend Harrison Keller (Chris Hanrahan) asks her out,[26] which brings Chloe into conflict with Mark, who disapproves of her meeting up with men for money.

[28] Speaking to Johnathon Hughes of the Radio Times, Pengilly explained that Shane actually asks Chloe for some information about her latest client, as he wants to do business with him.

[29] Pengilly later wrote that she and Johal thought it was "inappropriate" for Dipi to pin the blame on Chloe, while keeping Shane out of it, despite initially believing that he was cheating on her.

[30] While helping Pierce organise a wine tasting event, Chloe hugs him upon hearing that Tyler is being released, which leads to them having sex.

[31] She confides in Elly, who encourages her to speak to Pierce about their relationship, but Chloe chooses to keep the gifts and focus her attention on Tyler's imminent return.

Prior to the diagnosis, Chloe receives several letters and emails from a clinic in the United States, where she underwent some genetic medical trials in return for money.

While helping Pierce organise a wine tasting event, Chloe hugs him upon hearing that Tyler is being released, which leads to them having sex.

She confides in Elly, who encourages her to speak to Pierce about their relationship, but Chloe chooses to keep the gifts and focus her attention on Tyler's imminent return.

Chloe supports Kyle when she finds out he has cancer, they grow closer as friends since she can understand his fear about kids and dying as she has Huntington's disease.

Two years later, Chloe returns to Erinsborough to help run Lassiters, following the absences of Paul and the new co-owner's daughter Reece Sinclair (Mischa Barton).

Chloe experiences an increase in her Huntington's disease symptoms, and when Elly eventually joins her, she offers her the chance to leave their relationship before her condition worsens.

[49] In 2021, Chloe was placed sixth in a poll ran via soap fansite "Back To The Bay", which asked readers to determine the top ten most popular Neighbours characters.