[2] Choo Choo Soul began as a collaboration between video game developer Greg Johnson, recording engineer and sound designer Burke Trieschmann, and vocalist Genevieve Goings during dialogue recordings for ToeJam & Earl III: Mission to Earth, the first Xbox installment of the ToeJam & Earl video game franchise.

[3] Johnson created the concept and recorded melodies and accompaniment tracks onto his cellphone and e-mailed them to Treischmann to develop.

[4] The act debuted in 2004 as a 16-track CD Baby studio album that described itself as "kids' music that won't drive parents crazy."

Choo Choo Soul has including more premieres and has more dubs of the show than Hebrew, Spanish and Italian, including Hebrew dubs on Israeli Educational Television (now Kan Educational), the Spanish dub of the show between the airings on Univision blocks of Disney Junior shows and the Italian dubbed version of the show airing on Playhouse Disney (after reruns of Choo Choo Soul episodes airing on Disney Junior).

Choo Choo Soul has performed live on several occasions in 2007, including September and October shows at the Disney-MGM Studios theme park at Walt Disney World Resort in Florida, a performance at the Boston Globe Children's Book Festival in Boston, Massachusetts on September 15 and several shows in June 2009 at Sesame Place located near Philadelphia, Pennsylvania.