The B35 bus route starts at a cut-off lane on 39th Street slightly east of 1st Avenue in Sunset Park, near Industry City and the South Brooklyn Marine Terminal.

The B35 also offers daily Limited-stop service (instituted in September 2005) between McDonald Avenue in Kensington and the route's Brownsville terminus at Mother Gaston Boulevard throughout the day.

During PM rush hours, some local trips to Kings Highway originate at the Church Avenue subway station served by the 2 and 5 trains.

In 1975, the New York City Transit Authority began offering free transfers to it from the subway as a replacement for the Culver Shuttle.

Bus lanes for the B35 on Church Avenue between East 7th Street and Marlborough Road were put into effect in October 2019.