The church has historically maintained a strong relationship with the neighboring Washington & Jefferson College.

[2] The split, which saw 36 members leave the First Presbyterian Church to join the Second, was reportedly "attended with expressions of the best Christian feeling" between the congregations.

[2] In 1870, George P. Hays became "stated supply", while also serving as President of the neighboring Washington & Jefferson College.

[1] The new building at 65 East Beau Street was dedicated on March 6, 1887, with Hays returning to give the sermon.

[1] The building featured a Johnson Pipe Organ and a 450-seat auditorium with a groined ceiling and bowled floor and an adjacent lecture room.