Spirito Santo ('Holy Spirit') is a Catholic church located in Alcamo, in the province of Trapani, Sicily.

Documentation of the church's existence dates back to May 11, 1491, among the deeds of the notary Giacomo Gruppuso of Alcamo.

The church is on an isolated plot of land that is full of trees, and managed by the Rectors of the Confraternity of the Annunciation.

[1] In his work Discorso storico della opulenta città di Alcamo, the historian Ignazio De Blasi mentions a bill of sale concerning an enclosure in the districts of San Giovanni and Sant'Angelo, dated November 20, 1593, and a land concession dated August 17, 1549, among the deeds of the municipality of that period.

The shape of the building, the tempera painting, and the main areas exposed to the west, as in the nearby chapel of San Giovanni Battista, indicate that it was built in the same period.