[5] Techniques used for the identification of citrus psorosis virus include the enzyme-linked immunosorbent assay (ELISA) that uses a specific antibody to determine the antigen, polymerase chain reaction to amplify target sequences, and detecting nucleic acids by lateral flow microarrays.

Other methods commonly used are direct tissue blot immunoassays, fluorescence in-situ hybridization, immunofluorescence, and electron microscopy for viral pathogen detection.

The best management practices that can be implemented involve sourcing indexed and certified disease-free budwood, temporary recovery by pruning infected bark, host removal, and disinfection of machinery or equipment.

The grapefruit used for CPsV-EG isolatation was found to be free from CTV, CEVd and Spiroplasma citri by testing with DTBIA, tissue print hybridization and Diene's stain respectively.

A partial fragment of RNA3 (coat protein gene) of CPsV-EG (–1140bp and –571bp) was amplified by reverse transcription-polymerase chain reaction (RT-PCR) from grapefruit tissues using two sets primers specific CPsV (CPV3 and CPV4) and (PS66 and PS65) respectively.

The serological characters represented as the antigenic determinants of CPsV-EG isolate related to monoclonal antibodies specific CPsV strain where as appeared precipitation reaction by DAS-ELISA and DTBIA.

[8] CPsV-EG was detected on the basis of biological indexing by graft inoculation which gave oak leaf pattern (OLP) on Dweet tangor and serological assay by DAS-ELISA using Mab specific CPsV.

[9] In general CPsV-EG-infection affects the upper epidermis of the leaf which is composed of non-tabular parenchyma cells covered by a thin layer of cuticle.

The vein endings consist of a single trachoid[check spelling] strand of elongated parenchyma cells enclosed by the bundle sheath compared with healthy ones.