The Columbia Branch is a railway line in Boone County, Missouri.

Originally built by the Boone County and Jefferson City Railroad in 1867, today it is owned by the city of Columbia and operated by the Columbia Terminal Railroad.

The North Missouri Railroad was reorganized in 1872 as the St. Louis, Kansas City and Northern Railway, while the Boone County and Jefferson City Railroad itself reorganized as the Boone County and Boonville Railway in 1873.

[3] Finally, in 1902, the Wabash formed the Columbia and St. Louis Railroad as a vehicle to acquire the Boone County and Boonville Railway and the Columbia Branch outright.

[5] When the Norfolk Southern proposed the abandon the line in 1987, the city of Columbia acquired it and created the Columbia Terminal Railroad to serve freight customers along the route.