[1] They were located in Columbia, Tennessee,[1] and were named in reference to Columbia being known as the "Mule Capital of the World" and holding "Mule Day" celebrations since 1840.
[2] Their home games were played at Pillow Park[3] Columbia was previously represented by an unnamed team in the Tennessee–Alabama League from 1903 to 1904.
[1] The Mules lost their season opener against the Tri-Cities Triplets, 4–2, on May 16, 1921.
[5] They did not qualify for the championship playoffs as they did not win either half of the league' split season.
[5] The city did not field another team until a different Columbia Mules played in the Big South League in 1996.