The main line, formerly part of the Pennsylvania Railroad's "Panhandle," was acquired from Conrail in 1992.

Piney Fork Subdivision - Using Ohi-Rail trackage rights to North Apex, Ohio.

The company was acquired by Genesee & Wyoming in 2008 as part of its purchase of the Ohio Central Railroad System.

CSX and Norfolk Southern Railway have interchanges at Columbus, Ohio.

[3] The railroad received a $750,000 grant from the Ohio Rail Development Commission in May 2023 to support additional tracks in Newark Yard, the primary yard on the CUOH system.