The "LO" stands for "lolita only",[3] reflecting its focus on fictional young (or young-looking) girl characters.

When first published, Comic LO helped trigger a small boom in lolicon manga in the early 2000s.

[4] Comic LO was originally an extra issue of other erotic magazines, but it became independent on December 21, 2005.

[5] On May 22, 2010, the publisher put out an announcement to stop the illegal uploading of the magazine on its official website.

Amagappa Shōjogun, a manga artist for the magazine was told to draw more girls that look like 9 year olds as 8 was too young.