The Monasterio de las Descalzas Reales is a monastery located in Valladolid, in Castile and León, Spain.

This monastery is to be distinguished from a similarly named one in Madrid of the same Order (see Convent of Las Descalzas Reales).

[1] It has been based in Valladolid since 1552, when the community moved to its permanent home, and its dedication was changed to Our Lady of the Assumption by order of King Philip III of Spain, who also initiated a rebuilding program.

He was in charge of providing the convent with a home in Puerta del Campo, in the hope of definitively occupying the area in front of the chancellery.

In 1552, Fadrique Osorio of Toledo and his wife, Inés de Pimentel, Marquess of Villafranca, bought for the convent the Palace of Alonso de Argüello (secretary to the Majesty), the houses licensed to Galarza (of the council to His Majesty), and those licensed to Ortiz (professor of the University of Valladolid).