This special adaptation prevents water loss, since fewer blood vessels occur in the "co-ossified" (fused) area.

Cuban tree frogs are able to secrete a toxic mucus from their skin which can cause a fiery sensation if it makes contact with a person's eyes.

During the breeding season, the male will have a black nuptial pad on his hand or wrist that helps him hold onto the female during amplexus.

[14] The Cuban tree frogs' progressive colonization into the mainland of Florida is believed to be abetted by use of State Road A1A construction during the 1940s.

[5] The Cuban tree frog is known to hitchhike on shipments of potted plants,[18] vegetation, packaging,[17] boats, and other motorized vehicles.

[20] Cuban tree frogs are known to inhabit a variety of habitats, including estuaries, low-density suburban development, small towns, agricultural areas, particularly ones with exotic plants, and lowland forests and swamps.

[21] Within their habitats, they can be found in damp, shady areas, particularly around shrubs and trees,[9] by cisterns, rain barrels,[14] and buildings.

[22] It is inadvertently carried on vehicles or ornamental plants, spreading to new areas, and has been transported as far north and west as Saskatchewan, Canada.

This can be done, most humanely, through the application of Orajel to the abdominal skin, waiting fifteen minutes for the frog to be fully anaesthetized, and then placing it in a freezer for a few hours.

They feed readily on any animal they can fit in their mouths, which can result in cannibalistic behavior if frogs housed together have a significant size difference.

[8] The Cuban tree frog's colonization of Oahu is believed to be a result of accidental or deliberate release of pets.