[2] Commonly found in the central and southeastern United States, the frog lives in open canopy forests with permanent water sources and abundant vegetation.

[5] When defending its territory, the frog either emits aggressive call signals or resolves to grapple with intruders, seldom leading to injury or death.

[13] The American green tree frog is considered monotypic, but clinal variation has been observed from Florida north along the Atlantic coastal plain.

[4] American green tree frogs prefer to live in open canopy forests with a permanent water source and filled with plentiful vegetation.

[3] A growing number of American green tree frogs have experienced severe habitat loss primarily due to urbanization and destructive wildfires that can destroy forest canopy cover.

Since most amphibians have narrow habitat tolerances and migration constraints, American green tree frogs urgently need alternative shelters for survival as forest canopies recover.

In a study from Central Texas, scientists have tried to combat wildfire habitat loss by creating artificial shelters using PVC pipes.

[15] American green tree frogs will defend their mate calling sites against foreign rivals and invaders using aggressive interactions.

[6] American green tree frogs are insectivores, primarily consuming flies, mosquitoes, grasshoppers, cockroaches, spiders, beetles, and other small insects such as crickets and ants.

The same study showed "nearly 90% of Hyla cinerea prey were actively pursued", with the other 10% being "insects walking or close enough to be snatched up by the frog's tongue".

[18] Most American green tree frog females breed once per year, but some have multiple clutches in a single mating season.

In a Florida population, "advertisement calls of males were documented between March and September and pairs in amplexus were observed between April and August".

Consequently, male individuals experience intraspecific mating competition and often encounter immense pressure to produce unique call signals that are both attractive and audible to the limited number of available females.

Such challenges are further complicated by the rapid fluctuation of males within a chorus, the potential risk of increased exposure to predators, and sexual selection of specific call signals through female choice.

[24] Some male American green tree frogs will not emit or alter their advertisement calls and instead choose to remain silent.

Labeled as 'satellites', these frogs will wait to intercept the signals of nearby calling males and mate with approaching sexually active females through amplexus.

[25][26] As a tadpole, the American green tree frog is easily predated by sunfish, bass, and dragonflies, including both aeshnidae and libellulidae odonate naiads.

To combat predation, green tree frog tadpoles may increase hiding behavior while in water to avoid capture.

[28] Contrary to most amphibians, the American green tree frog is not easily susceptible to the Batrachochytrium dendrobatidis (Bd) pathogen and the disease chytridiomycosis.

These observations suggest a possible mechanism dictating vocalization and the alternation between calling and non-calling behavior in the green tree frog.

[30] American green tree frogs are popular pets because of their small size, appearance, and the undemanding conditions needed to take care of them.

Synthetic compounds such as polychlorinated biphenyls are found in many pesticides and pollute the green tree frog's aquatic habitats.

Because the frog's skin is thin and permeable, synthetic compounds absorb easily upon contact, making the species a viable variable to measure contamination.