Subway trains started to run on the Culver Line on May 30, 1931, when the Nassau Street Loop was completed.

Rush hour trains ran express on the BMT Fourth Avenue Line.

Trains operating in the reverse-peak direction ran express between Ninth Avenue and Kings Highway.

Saturday morning rush hour Culver-Nassau Street Express and Culver Shuttles were discontinued on June 24, 1950.

As a replacement, the transit authority offered free transfers to the parallel B35 bus route.