The series centers around three children from Earth: Jackie, Matt and Inez, who are brought into Cyberspace, a digital universe, in order to protect it from the villainous Hacker (Christopher Lloyd).

In Cyberspace, they meet Digit (Gilbert Gottfried for the first thirteen seasons, later Ron Pardo as of the fourteenth), a "cybird" who helps them on their missions.

Marbles drained Hacker's battery and banished him to the Northern Frontier, where he formulated a plan to launch a computer virus that would attack Motherboard.

When Jackie, Matt and Inez interact with a library map in the real world, they accidentally allow Hacker access to Motherboard and she becomes infected with the virus.

[13] Cyberspace consists of planet-like bodies called Cybersites, with each site having a theme such as Ancient Egypt, the American frontier, Greek mythology and amusement parks.

Cyberchase has had many guest stars over the years, including Jasmine Guy, Tony Hawk, Al Roker and Geoffrey Holder.

These "For Real" segments are broadcast exclusively on PBS in the United States where the program runs longer and fills the time gap where there might be breaks for "commercials".

The reviewer, Joly Herman, commented that "the adventures aren't scary, violent, or sexually inappropriate" but "require fortitude and brain power".

Proposed discussion points sprouting from the series include "Can your child take the skills learned in each episode and figure out how to apply it to everyday life?"

The review commended the series for its accessibility: "Children can easily learn from the example in the cartoon story" and praised the "Cyberchase For Real segment that follows each episode".

[26] ^2007 Outstanding Broadband Program nominees : Frances Nankin, Sandra Sheppard, Ellen Doherty, Jill Peters, Bob Morris, Suzanne Rose, Michelle Chen, Elizabeth Hummer, Arash Hoda, Anthony Chapman, David Hirmes, Bianca DeGroat, Matthew A. Wilson, George Arthur Bloom[25] ^2008 Outstanding New Approaches nominees : Sandra Sheppard, Frances Nankin, Ellen Doherty, Jill Peters, David Hirmes, Kelly Lafferty, Michelle Chen, Tanner Vea, Bob Morris, Matthew A. Wilson[37] ^2009 Outstanding New Approaches nominees : Sandra Sheppard, Frances Nankin, Ellen Doherty, David Hirmes, Tanner Vea, Denyse Ouellette, Gilbert Gottfried, Phil Williams, Robert Tinkler[38] ^2012 Outstanding New Approaches nominees : Frances Nankin, Sandra Sheppard, Jill Peters, Marj Kleinman, Ellen Doherty, Kristin DiQuollo, Michelle Chen[39]