The Czech Republic competed at the 2000 Summer Olympics in Sydney, Australia.

The following is the list of number of competitors participating in the Games: * Six footballers (Erich Brabec, Petr Čech, Martin Čupr, Marcel Lička, Jan Polák and Luděk Stracený) were substitutes, but did not start.

Head coach: Karel Bruckner The Czech Republic had one rower participate in one of the fourteen events in 2000.

[1] Qualification Legend: FA=Final A (medal); FB=Final B (non-medal); FC=Final C (non-medal); FD=Final D (non-medal); FE=Final E (non-medal); FF=Final F (non-medal); SA/B=Semifinals A/B; SC/D=Semifinals C/D; SE/F=Semifinals E/F; QF=Quarterfinals; R=Repechage The Czech Republic captured one of the first Olympic triathlon medals when Jan Řehula passed ten other competitors during the final phase of the race to win the bronze medal.

Two other Czech triathletes finished the race, while a fourth competitor withdrew during the cycling phase.