In 1920 the Faculty of Agriculture and Forestry was established, and in 1952 the faculty became an independent institution, University College of Agriculture in Prague (Czech: Vysoká škola zemědělská v Praze; VŠZ).

The Faculty of Forestry, established in 1952, was part of ČVUT until 1959, then a part of the agricultural university until 1964, when it became the independent Institute of Forestry Science (Czech: Vědecký lesnický ústav).

During communist rule in Czechoslovakia, the university was lavishly funded as part of state support of collectivised agriculture.

From 1952 to 1980 the master's degree offered by the university lasted five years.

The Secretary of the Interfaculty Committee Agraria is located at CULS.