All of the varsity Patriot athletic teams compete at the Division II level with the exception of the baseball team, which plays in Division I. DBU Athletics also sponsors five club programs including; cheer, dance, bass fishing, lacrosse, and ice hockey.

The school's athletic department features a Christ-centered discipleship program, as a part of the mission to develop "Champions for Christ".

DBU athletics also launched the Global Sports Mission Initiative in 2007 to allow student-athletes the opportunity to share the love of Christ, while using their God-given athletic talents and gifts to minister and spread the Word of God abroad.

The Global Sports Mission Initiative was created as a vision by Patriot athletics and the DBU Global Missions Department and has included trips to Guatemala, Peru, Dominican Republic, South Korea, England, Brazil, Chile, Liberia, Scotland, China, Honduras, and Curacao, with plans for more trips in the near future.

The Cheer program has won three straight NCA National Titles in Daytona Beach, Florida, in 2021, 2022, and 2023.