She came to prominence in the 1980s in the feminist political theatre troupe Split Britches, which she co-founded with Lois Weaver and Peggy Shaw.
Literary theorist Lynda Hart edited and wrote a commentary on each piece.
[3] In the 1990s, Margolin participated in the Zale-Kimmerling Writer in Residence program at Tulane University in New Orleans, LA.
She later donated a large quantity of her personal materials such as journals, manuscripts, newspaper articles, flyers, ephemera, poetry, and correspondence to the Newcomb Archives at Tulane in August, 2018, forming the Deb Margolin Collection which spanned from the years of 1970 to 2016.
In the spring of 2020, an exhibit was later shown at Tulane University entitled "Deb Margolin's Performance Composition: Writing and Embodying" which focused on Margolin's process of performance composition by displaying her notes and writing (both handwritten and typed) from her time as a university professor, actor, and playwright.