Glick owned and managed a small printing business in TriBeCa before becoming deputy director of general services at the New York City Department of Housing, Preservation, and Development, where she worked until May 1990.

[1] Glick is a member of the Vote Blue Coalition, a progressive group and federal PAC created to support Democrats in New York, New Jersey, and Pennsylvania through voter outreach and mobilization efforts.

[7] Glick's legislative victories include the passage of the Sexual Orientation Non-Discrimination Act (SONDA), which was finally signed into law in December 2002.

Glick was the lead sponsor of the Reproductive Health Act, to codify and expand protections granted in Roe v. Wade in New York State, which was signed into law in January 2019.

Deborah Glick supported the legalization of gestational surrogacy in the State of New York with some minor adjustments and changes to the bill to protect the surrogate and the parties involved in regards to monetary compensation.

Governor Andrew Cuomo signed it into law in 2021, effectively repealing the ban on the medical procedure with monetary compensation for the pregnant women involved.

[9] Glick believes in family planning methods and stated on Twitter in October 2021 that there is a problem called overpopulation of people on the planet, causing damage to the Earth.

These include the Veterinary Emergency Response and Mobility Act, which allows out-of-state veterinarians and veterinary technicians to provide assistance in New York during declared disasters and high-volume animal cruelty investigations; an act to prohibit the importation, possession, sale, or release of Eurasian boars in New York State; and a law prohibiting the online shooting of animals or targets.

She has also fought for increased funding for animal shelters and was twice successful in allocating a $5 million capital matching grant to humane societies across the state.

Glick has also been an ardent supporter of the rights of student workers, such as graduate teaching and research assistants, to collectively bargain through establishing trade unions or labor organizations.