Joel, a minister like his deceased father, was raised by his religious grandmother after both of his parents were killed in a car accident.

Joel becomes disillusioned with Christianity and decides to take a trip to New York to visit K. While awaiting Joel's arrival, K visits a bar and meets an attractive woman, who is known by the bar patrons and bartenders as an unusual "Temptress" who brings a lot of men home with her, but unbeknownst to them, she kills the men after they engage in sex.

K's friend Dougy, a police officer who deals in supernatural cases, notices the men's disappearances after they leave with the Temptress.

The next morning, Joel prepares to go out on a date with the Temptress, who arrives at the house, still pretending she does not know K. K, frustrated over having his feelings manipulated, calls her out over the pretense until he notices she doesn't have a reflection in the mirror.

K and Dougy go and see Madam Sonya, a fortune teller who reveals the woman is a succubus who murders men who are unfaithful and tempted by her.

Meanwhile, K and Dougy go to the bar, where the Temptress is, and tell the bartender to put blessed holy water in her drink and then to get away fast.

Later, Dougy arrives at the bar in a limo driven by K. He takes a seat inside, where a woman lights his cigarette.

Quoting an earlier suggestion from the Temptress, it’s revealed that he has transformed into an incubus along with K. The film concludes with a recurring dream sequence of Joel running through the smoky streets of New York.

James Bond III directed Def by Temptation, his debut feature film, in four weeks with a $5 million budget.

"[6] Writing for the New York Times, Janet Maslin described the film as "remarkably tame" for a Troma Entertainment production and praised the performances of the cast, but criticized the special effects, stating "[Bond's] film is more memorable for its sheer ambition and occasional flashy style than for its execution.