Following his role as a United States Naval Officer and after completing graduate studies at his OSU alma mater, he again worked with the Navy as a civilian U.S. Government Nuclear Engineer.
Within two years, Dennis Chamberland had designed and built the NASA Scott Carpenter Space Analog Station (SCSAS), a two-man undersea habitat.
The link schedule also included linkage with a NASA team of investigators in isolation at the Johnson Space Center, where they were testing long term, advanced life support systems.
On board the Scott Carpenter Station, investigators also tested space life support systems for growth of plants in remote and extreme environments.
Visitors to the seafloor Scott Carpenter Station during the Challenge Mission included motion picture director and writer James Cameron, who conferenced with students north of the Arctic Circle in Pond Inlet, Nunavut, via satellite phone, as well as producer Eugene "Rod" Roddenberry II, and Tom Whittaker – the first disabled person to climb to the summit of Mount Everest.
Dennis Chamberland began his third attempt at launching the first permanent undersea colony in the Gulf of Mexico in Florida Bay in 1998–1999 called the Trident Project.
He was featured in National Geographic’s documentary series Naked Science "City Under the Sea" [1] episode and Motherboard’s "The Aquatic Life of Dennis Chamberland" While at NASA, Dennis Chamberland was one of the longest serving NASA Chairmen of any Center's Institutional Animal Care and Use Committees, holding the position for 14 years and reviewing every vertebrate animal research payload that flew on Shuttle, or to Mir Space Station or the ISS from Shuttle.
Chamberland was also a Principal Investigator for the landmark scientific study conducted by a team from the John F. Kennedy Space Center, Brookhaven National Laboratory, McKnight Brain Institute at the University of Florida, and Cold Spring Harbor Laboratory that inquired into the chronic neurological effects of galactic space radiation related to a crewed mission to Mars utilizing the Brookhaven Collider-Accelerator.
In 2022 Dennis Chamberland published the two-volume book titled "Departing Earth Forever" in which he projected the end of the obsolete Apollo model of human space exploration and its replacement.
These diseases are expressed in humans who are exposed to full spectrum cosmic radiation for an unknown duration, perhaps in exposures in as little as a week, according to some early results of research on NASA Apollo Lunar astronauts.