It is managed by Natural England and has a permanent staff of wardens who carry out conservation works and ensure the dales are usable for recreation.
The dales are a mixture of grassland, woodland and scrub; some also have streams (generally winterbournes).
They are a haven for biodiversity and many Sites of Special Scientific Interest (SSSI) lie partly or wholly within the reserve boundaries.
Dippers are common along the River Lathkill, and ravens and buzzards are regularly seen around the dale.
The Monyash end of Lathkill Dale is well known for the flower Jacob's ladder (Polemonium caeruleum), which is common in gardens but rare in the wild.