Desmognathus organi can be identified by its small size, averaging only 40–60 mm total length, and its distinct herringbone pattern on the dorsal side and copper colored eyelids, which are traits only shared with D. wrighti.

Characteristic of the Desmognathus species, there is a light colored eye stripe to the jaw, and the hindlimbs are longer and more robust than the forelimbs.

Populations of Desmognathus organi can be found in high elevation spruce-fir and hardwood forest habitats in the Blue Ridge Mountains.

Its sister species, D. wrighti, occurs at similar elevations and habitats south and west of the French Broad to the Georgia state line.

D. organi have been reported climbing trees as high as 2 meters off the ground, and this is believed to be a method for catching prey or escaping predation.

This is how they achieve internal fertilization, and the female will lay a clutch averaging 3-10 eggs in a seepage area or wherever she can find moist ground and stay nearby to protect them.