Yūki Anzai, a half human, half-vampire, is a member of the Metropolitan Police Department's Public Safety Division 5 and is in charge of cracking down on vampire crimes.

Devils' Line, written and illustrated by Ryo Hanada, was serialized in Kodansha's seinen manga magazine Monthly Morning Two [ja] from March 22, 2013, to December 22, 2018.

[16] A sequel manga series, titled Devils' Line II: Trigger (デビルズラインII［逆襲］, Debiruzu Rain Tsū Torigā), began serialization in Morning Two on January 20, 2022.

[16] The series is directed by Hideaki Nakano at animation studio Platinum Vision, with Ryouma Mizuno serving as assistant director.

[56] Sentai Filmworks have licensed the anime in North America, the British Isles, Australasia, and other regions, and streamed the series on Hidive.