[5] The male advertisement call is distinctive and consists of a single note about 0.06 seconds in duration.

Its dominant frequency is about 4400 Hz, higher than advertisement calls of other Diasporus species.

[5] It inhabits humid tropical and sub-Andean forest at elevations of 280–1,200 m (920–3,940 ft) above sea level.

Specimens have been found on a tree branch, on a trail, in a trashcan, and inside of a petiole of Xanthosoma sagittifolium.

[4] Although these frogs appear rare based on museum collections and sightings during field expeditions, acoustic surveys suggest that they can be fairly abundant inside forest patches.