He is among the founders of the Compagnie van Verre, the United East India Company (VOC), and the Amsterdam Exchange Bank.
[4] He and his brother Hendrick traded in leather, grain and precious stones, primarily in the Levant and cities on the Baltic Sea.
[5] In 1595, he organized an expedition with Isaac le Maire to get salt in Setúbal and ship it to Archangelsk In 1602 he was one of the founders of the Dutch East India Company, and served as one of the first directors.
In 1612 Van Os acquired more than one-seventh of the land in Beemster and in the Purmer[10] His portrait is in the Stedelijk Museum Alkmaar.
[11] His son, Dirck van Os II (1590–1668), served from 1618–1666 as the dijkgraaf of Beemster, and was the subject of a Rembrandt portrait currently owned by Joslyn Art Museum in Omaha, Nebraska.