In particular, the Achillea Dispositio stipulated that the Mark Brandenburg was to remain undivided and to be inherited in its entirety by the eldest son (at Albrecht Achilles death, that would be John Cicero).
Strictly speaking, at the time the Dispositio Achillea was written, it only defined the division of the Margraviates among the sons of the Margrave of the day.
The central element of Achillea Dispositio was the principle of the indivisibility of the Mark Brandenburg, which it made part of the Hohenzollern House law, and not—as previously—only a clause in the Golden Bull.
At the same time, it provided the cornerstone for a development that eventually lead to progressive separation of the Mark Brandenburg from the Hohenzollern stem lands in Franconia.
It created the two territories of Brandenburg-Ansbach and Brandenburg-Kulmbach, which were only reunited with the Mark's successor state of Prussia in 1791.