It has between two and six narrowly linear to thread-like leaves and up to three yellow flowers, usually with dark red markings.

There are two smooth calli ridges outlined with red near the base of the labellum.

[6] The specific epithet (segregata) means "separate", or "set apart", referring to its differences from D. septentrionalis, that grows in a similar area.

[4] Northampton bee orchid grows in winter-wet areas, near the edges of temporary lakes and around rocks between Eneabba and Kalbarri in the Avon Wheatbelt, Geraldton Sandplains and Swan Coastal Plain bioregions of south-western Western Australia.

[2][3][4] Diuris segragata is listed as "not threatened" by the Western Australian Government Department of Biodiversity, Conservation and Attractions.