[1] In 1973, when the National Collegiate Athletic Association divided into a three-division format, the Dixie Conference left the NAIA and moved into the non-scholarship NCAA Division III.

Three colleges announced plans to join the USA South Conference beginning in the 2012–13 season while one departed the league.

Shenandoah University left the USA South on the above date, to become a full member of the Old Dominion Athletic Conference (ODAC), a league in which it held associate membership in several sports.

[9] Later changes to the USA South membership came on March 8, 2021, when Averett University revealed its intention to leave the USA South and join Ferrum College in the ODAC as a full member in the 2022–23 academic year,[10] and on November 22, when Belhaven University, then a member of the American Southwest Conference, was at first accepted for membership in the conference also for 2022–23.

Belhaven had been originally announced as an incoming USA South member for 2022, but instead opted to join the CCS for that school year.