It runs 108 kilometres (67 mi) from Krems an der Donau to Mauthausen, near St. Valentin.

The line is no longer contiguous; 21.9 kilometres (13.6 mi) in the middle between Weins and Weitenegg [de] has been abandoned.

From there, the line runs 38.3 kilometres (23.8 mi) west along the north bank of the Danube to Weitenegg [de].

That line is now abandoned north of Mauthausen; the remainder runs south, crossing the Danube to reach St. Valentin [de].

[2] On the western end, Austrian Federal Railways (ÖBB) operates hourly Regionalzug services between St. Nikola-Struden and St. Valentin [de].