[2] Allopatric speciation has been induced by reproductive isolation in D. pseudoobscura after only eight generations using different food types, starch and maltose.

[4] In the D. pseudoobscura population, some males have a harmful chromosome called sex ratio (SR), where an inactive Y-chromosome is transmitted.

[8] Males D. pseudoobscura ejaculate more sperm than any other Drosophila species, and it provides important nourishing factors to females and their offspring.

Densely populated areas may have lower rates of polyandry due to environmental restraints such as geographic location and limited resources.

[9] Therefore, in population dense areas, polyandrous behavior may actually be a fitness consequence since the environment significantly controls the number of offspring that survive.

[10] Ehrman et al. made extensive studies of D. pseudoobscura males' mating pheromones – both as isolate and as whole body extracts – in the 1970s.