DuBose Ausley (born May 13, 1937) is an attorney in Tallahassee, Florida, USA.

Ausley's great-grandfather was Alexander McSwain, a member of the Florida House.

His father was Charles S. Ausley, a member of the Florida Senate.

His daughter is Loranne Ausley, member of the Florida House of Representatives (2000–2008, 2016–2020.)

He served on the United States Circuit Judge Nominating Commission.