A former Ford Supermodel of the World runner-up, Du made her runway debut in spring 2006, walking for prominent designers such as Valentino (opening and closing his bridal show), Jean-Paul Gaultier, Roberto Cavalli and Chanel.

With multiple bookings being a rare accomplishment, particularly for a new face, Style.com the online home of Vogue and W magazine, declared her one of the top ten models to watch for the fall 2006 season.

Du peaked at number 14 on model ranking site Models.com and in recent seasons has been featured in advertisement campaigns for Giorgio Armani, Esprit, Anna Sui, Uniqlo, David Yurman and Yue Sai, a Chinese expansion of L'Oréal.

Du has appeared in editorials for Allure, Harper's Bazaar, Elle, V, i-D and the Italian, American, Chinese, French, Taiwanese, Korean, and British editions of Vogue.

Du Juan made her acting debut in the American Dreams in China, a highly-grossing film directed by Peter Chan in which she played Su Mei, the only female role in the movie.