Yoon was born in Yonggang-gun, Pyeongannam-do, He graduated from Pyeongyang Public High School in 1938 and Gyeongseong Medical College in 1942.
During the Korean War, he participated in the reconstruction of Paik Hospital together with Dr. Huigyu Kim and Dr. Nakhwan Paik, and he played a leading role in founding a blood bank as he was working for the field hospital of the 24th Division of the US Army.
His experience at this time convinced him of the need of advanced medical skills, so he went abroad and studied at Bridgeport Hospital in the U.S. from July, 1954 to August, 1956.
In addition to managing various institutions, Yoon also put in a great deal of effort to train talent in the field of medicine.
He took over the campus of the Sacred Heart Women's College in Chuncheon in 1981, and established the Ilsong Education Foundation in January, 1982, serving as its first chairman of the board of the trustees.